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在分子生物學(xué)實驗中，PCR（聚合酶鏈?zhǔn)椒磻?yīng)）是技術(shù)之一，而PCR耗材的穩(wěn)定性直接影響實驗結(jié)果的可靠性。今天為大家介紹一款“細(xì)節(jié)控"實驗室的寶藏耗材——USAscientific貨號1402-4700，0.2ml PCR 8-tube FLEX-FREE strip attached clear flat caps natural 八聯(lián)排無核酸酶薄壁PCR管（以下簡稱“1402-4700八聯(lián)排管"）。它憑借多項創(chuàng)新設(shè)計，成為PCR實驗的“效率加速器"。
產(chǎn)品詳細(xì)介紹：從結(jié)構(gòu)到材質(zhì)的“硬核配置"
1402-4700八聯(lián)排管是一款專為PCR實驗優(yōu)化的耗材，核心設(shè)計圍繞“精準(zhǔn)、便捷、安全"展開：
- 品名：0.2 ml PCR 8-tube FLEX-FREE strip attached clear flat caps natural 八聯(lián)排無核酸酶薄壁PCR管(0.2ml)
- 結(jié)構(gòu)設(shè)計：采用經(jīng)典8聯(lián)排條帶設(shè)計，每條包含8個獨立0.2ml PCR管，管體與平蓋通過“FLEX-FREE"彈性連接，既保證運輸時的穩(wěn)定性，又支持單手輕松掰取單管，避免傳統(tǒng)連蓋管“暴力拆條"的麻煩。
- 材質(zhì)與工藝：管體采用醫(yī)療級聚丙烯（PP）材質(zhì)，經(jīng)伽馬射線輻照滅菌及無核酸酶處理工藝，確保無DNase/RNase殘留（第三方檢測報告驗證）；透明平蓋采用相同材質(zhì)，與管體緊密貼合，耐高溫（-20℃~121℃），適配常規(guī)PCR熱循環(huán)儀。
- 參數(shù)規(guī)格：單管容量0.2ml（±0.5%誤差），管壁厚度僅0.15mm（薄壁設(shè)計），外徑12mm，高度47mm，符合主流PCR儀（如ABI 7500、Bio-Rad CFX系列等）的孔板適配要求。
核心優(yōu)勢：為什么它是PCR實驗的“優(yōu)選"？
1. 無核酸酶污染風(fēng)險：通過嚴(yán)格的去核酸酶工藝（輻照+化學(xué)處理），消除管壁殘留的核酸酶，降低實驗假陽性概率，尤其適用于高靈敏度實驗（如qPCR、痕量DNA擴(kuò)增）。
2. 薄壁設(shè)計，提升擴(kuò)增效率：0.15mm超薄管壁導(dǎo)熱更快，減少熱循環(huán)儀升降溫時間（實測可縮短10%-15%擴(kuò)增時間），同時減少溫度梯度差異，提高擴(kuò)增一致性。
3. FLEX-FREE結(jié)構(gòu)，操作更便捷：連排管體通過彈性連接固定，既避免運輸脫落，又能單手掰取單管，無需剪刀或暴力拆分，減少耗材浪費，尤其適合高通量實驗場景。
4. 透明平蓋，觀察與密封兼顧：平蓋材質(zhì)高透光，可直接觀察管內(nèi)反應(yīng)液體積及顏色變化；同時平蓋與管體緊密貼合，經(jīng)壓力測試（500g壓力）無液體泄漏，有效防止氣溶膠污染，適配自動化移液設(shè)備。
5. 全場景適配，兼容性強(qiáng)：無論是常規(guī)PCR、qPCR、逆轉(zhuǎn)錄PCR，還是基因測序、克隆驗證，1402-4700八聯(lián)排管均表現(xiàn)穩(wěn)定，是實驗室日常PCR實驗的“萬能搭檔"。
常見疑問與解答
Q1：產(chǎn)品真的“無核酸酶"嗎？如何驗證？
A：是的。1402-4700八聯(lián)排管采用伽馬射線輻照滅菌聯(lián)合無核酸酶處理工藝，出廠前經(jīng)第三方檢測（如Taq酶消化實驗），確認(rèn)無DNase/RNase殘留，可提供完整質(zhì)檢報告。
Q2：薄壁設(shè)計會不會容易破裂？
A：不會。管體采用高韌性醫(yī)療級PP材質(zhì)，雖壁厚僅0.15mm，但抗沖擊性強(qiáng)（經(jīng)-20℃低溫脆化測試無破裂），可承受121℃高壓滅菌（15分鐘）及反復(fù)熱循環(huán)，耐用性優(yōu)于普通厚壁管。
Q3：透明平蓋的密封性如何？會不會漏液？
A：平蓋與管體通過模壓工藝緊密結(jié)合，經(jīng)壓力測試（500g靜置30分鐘）無液體滲漏，且表面光滑無凹陷，可有效防止氣溶膠污染，適配移液槍、自動化工作站等設(shè)備。
Q4：是否適用于所有品牌PCR儀？
A：是的。管體尺寸嚴(yán)格符合國際標(biāo)準(zhǔn)（直徑12mm，高度47mm），兼容主流品牌熱循環(huán)儀（如ABI、Bio-Rad、Eppendorf、Roche等），無需額外調(diào)整孔板適配器。
Q5：可以高溫滅菌嗎？滅菌后性能會變化嗎？
A：支持121℃高壓滅菌（15分鐘），滅菌后管體無變形、無材質(zhì)老化，無核酸酶活性殘留，不影響后續(xù)實驗性能。
Q6：與普通八聯(lián)排管相比，優(yōu)勢在哪里？
A：普通八聯(lián)排管可能存在核酸酶污染風(fēng)險（未做去酶處理），且多為厚壁設(shè)計（影響擴(kuò)增效率）；此外，普通連蓋管拆條時易斷裂，浪費耗材。1402-4700八聯(lián)排管通過無酶處理、薄壁設(shè)計及FLEX-FREE結(jié)構(gòu)，綜合解決了這些問題。
Q7：包裝規(guī)格是多少？
A：每盒含100條八聯(lián)排管（即800個單管），每條獨立塑封包裝，方便取用和存儲，避免交叉污染。
Q8：存儲條件有什么要求？
A：未開封時建議常溫（15-30℃）干燥保存，避免陽光直射；開封后需密封保存，建議6個月內(nèi)用完，以保證最佳性能。
Q9：保質(zhì)期多久？
A：未開封狀態(tài)下保質(zhì)期為2年（自生產(chǎn)日期起算），產(chǎn)品包裝標(biāo)注具體生產(chǎn)日期及有效期。
Q10：是否兼容qPCR染料（如SYBR Green）？
A：是的。管體材質(zhì)高透明、無熒光淬滅，可清晰觀察qPCR反應(yīng)體系的熒光信號，適用于SYBR Green、TaqMan等主流熒光染料。
（注：本文內(nèi)容基于品牌公開資料及行業(yè)常規(guī)信息整理，具體以品牌信息文檔為準(zhǔn)。）
USAscientific熱賣產(chǎn)品
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貨號 | 品名 | 規(guī)格 | 品牌 |
1402-9200 | TempPlate® Semi-Skirted 96-Well PCR Plate, Straight Skirt, 0.2 mL | 5 sleeves of 2 (10) | USAscientific |
1402-2900 | TEMPASSURE 0.2 ML PCR PULL-APART 8-TUBE STRIPS, ATTACHED INDIVIDUAL DOME CAPS | 120 strips of 8 (960 tubes/caps) per box | USAscientific |
1402-2400 | TEMPASSURE 0.2 ML PCR PULL-APART TUBE STRIPS WITH DOME CAP STRIPS | 80 strips of 12 tubes (960 tubes) | USAscientific |
2932-0110 | Cold Storage Foil | 50 sheets | USAscientific |
1180-1840 | TipOne® RPT Filter Tips | 10 racks of 96 (960) | USAscientific |
1181-3810 | 10ul TipOne RPT filter tip | Case of 960 | USAscientific |
1182-1830 | FILTER TIP ST 1000UL XL TIPONE | 10 racks of 96 (960) | USAscientific |
1180-8810 | TipOne RPT Filter Tips | 10 racks of 96 (960) | USAscientific |
1183-1810 | 20UL TIPONE RPT FLTR TIP 960PK | Pack of 960 | USAscientific |
1180-1810 | RPT 1-20UL FILTER TIP 960/CS | Case of 960 | USAscientific |
1412-0000 | 1.2 mL Tube, Individual, Non-Automation Rack | 1000/Pack | USAscientific |
1180-3810 | TIPONE® RPT FILTER TIPS | Case of 960 | USAscientific |
91886775 | USA Scientific Inc Titer-Tops film, pierceable, sterile, 50/bx | Pack of 50 | USAscientific |
1884-2410 | 0.2 mL deep 384-well PlateOne polypropylene plate, square wells, natural, individually wrapped | 40/pack | USAscientific |
1402-2300 | TEMPASSURE PCR FLEX-FREE8-TUBE STRIPS，ATTACHEDINDIVIDUAL OPTICAL CAPS | 0.1ml | USAscientific |
2381-5048 | 81-place color-coded box, clear lid, mixed colors | Pack of 5 | USAscientific |
1111-3240 | TIPONE® PIPETTE TIP STACKS，10 µL natural graduated | 10 stacks (9600 tips) | USAscientific |
1111-0200 | TIPONE PIPETTE TIPSTACKS ，200 µL natural | 1 stack (960 tips) | USAscientific |
1111-3200 | TIPONE PIPETTE TIPSTACKS， 10 µL natural graduated | 1 stack (960 tips) | USAscientific |
1212-8000 | 1.1 ml tubes in strips of 8, natural, polypropylene, bulk, 120 strips （960 tubes） | 960 tubes | USAscientific |
1412-8410 | 1.2 ML DILUTION TUBES IN STRIPS OF 8 | RACK (10 RACKS OF 96) | USAscientific |
1415-2500 | 1.5 ML COPOLYMERMICROCENTRIFUGE TUBE | 500/bag | USAscientific |
2920-0000 | Microplate Adhesive Film | 100 sheets/box | USAscientific |
2344-1000 | TOTALLY TUBUL AR RACK SYSTEM | 1set | USAscientific |
9124-0100 | CoolSafe Block 96 wells Chamber | 0.2 mL/96-well PCR | USAscientific |
1420-9701 | SCREW CAP MICROCENTRIFUGE TUBES, SELF-STANDING | ea | USAscientific |
1183-1740 | TIPONE RPT FILTER TIP REFILLS | 100ul | USAscientific |
1412-1000 | 1.2 ml tube | 1000 個/包 | USAscientific |
1402-4708 | TEMPASSURE PCR FLEX-FREE 8-TUBE STRIPS, ATTACHED INDIVIDUAL OPTICAL CAPS | ea | USAscientific |
2380-1008 | 80-place tube rack, mixed colors, PP, 7 racks | 7 racks | USAscientific |
9187-1708 | USA Scientific Inc Cryo-Babies, 1.28 inch x 0.5 inch, assorted colors, on sheets | 85/sheet, 1700/box | USAscientific |
1122-1760 | TipOne 1000 μl XL graduated, refill, 40 cassettes of 96 tips | 96tips | USAscientific |
1120-8780 | TipOne 200 μl graduated filter tip refill 8-unit case, sterile, 7680 tips | 7680tips | USAscientific |
1120-1760 | TipOne 100 μl filter tip refill 8-unit case, sterile, 7680 tips | 7680tips | USAscientific |
1120-1880 | TipOne 20 μl filter tip 8-unit case, sterile, 7680 tips | 7680tips | USAscientific |
1121-3880 | TipOne 10 μl filter tip 8-unit case, sterile, 7680 tips | 7680tips | USAscientific |
1420-9710 | 2.0 Self standing Microcentrifuge tube, 500/pack Sterile | 500/pack | USAscientific |
1075-0110 | 5mL serological pipette | 200.pack | USAscientific |
1420-9410 | TETHERED SCREW CAP MICROCENTRIFUGE TUBES, SELF-STANDING | 100/pack | USAscientific |
9123-6100 | Breathe-Easy film, sterile | ea | USAscientific |
2998-6550 | LIGHT REFLECTING FILM | 50/box | USAscientific |
1405-4707 | 0.5 ml screw cap tube amber | 500/pack | USAscientific |
1420-9709 | 2.0 ml self-standing screw cap tube, black | 500/pack | USAscientific |
1475-0511 | 15mL Conical Tubes | 500 pack | USAscientific |
1071-0810 | 10 ml serological pipet, sterile | 100pack | USAscientific |
1620-2700 | SEAL-RITE® 2.0 ML MICROCENTRIFUGE TUBES | 500/pack | USAscientific |
1500-1211 | 50 ml conical screw cap tubes | 25 bags of 20 | USAscientific |
1420-9700 | 2.0 ml self-standing microcentrifuge tube, graduated, natural with natural cap | 500/pack | USAscientific |
1111-2820 | TipOne® Natural, Graduated, Racked Pipette Tips, 101-1001ul | 960 tips | USAscientific |
1402-3900 | TEMPASSURE PCR PULL-APART 8-TUBE STRIPS, ATTACHED INDIVIDUAL OPTICAL CAPS | 120 strips of 8 (960 tubes/caps) per box | USAscientific |
9138-2750 | Direct Thermal Cryo-Tags®，SIZE: 1.5" × 0.5"，COLOR: White | ea | USAscientific |
2920-0010 | MICROPLATE SEALING FILM Standard cut, sterile | 100 sheets | USAscientific |
9187-2016 | TOUGH-TAGS LABELS ON SHEETS | SIZE: 1.28" × 0.5" White | USAscientific |
9185-2000 | TOUGH-SPOTS LABELS ON SHEETS | SIZE: 1/2" diameter，White | USAscientific |
1111-2021 | 1000 µL blue graduated | 1000 tips/bag | USAscientific |
2530-1102 | Thermal-Lok 2-Position Dry Heat Bath, Includes 2 Blocks | 2 Blocks | USAscientific |
1833-9600 | PLATEONE:emoji: 96-WELL POLYPROPYLENE PLATE | Conical (V) Non-sterile | USAscientific |
1306-2510 | CHANNELMATE RESERVOIR SYSTEM | 25ml | USAscientific |
2939-6100 | SINGLE TAB SEALING FOIL, INSET CUT | 100/box | USAscientific |
1420-9799 | 2.0 ml self-standing screw cap tube, mixed color caps | 500/pack | USAscientific |
1011-8000 | 200 μL LARGE ORIFICE TIP | 1000 tips/bag | USAscientific |
1111-0240 | TIPONE PIPETTE TIP STACKS (200 uL natural) | 10 stacks (9600 tips) | USAscientific |
3823-3412（ Non-sterile） | 12-WELL V-BOTTOM RESERVOIR；3ml | Non-sterile 50/pack | USAscientific |
1110-1240 | 1-200ul tip racks (5 stacks, no filter) Natural； 10 stacks (9600 tips) | 10*5 stacks (9600 tips) | USAscientific |
1111-0246 | 1-200ul tip racks (5 stacks, no filter)Yellow； 10 stacks (9600 tips) | 10*5 stacks (9600 tips) | USAscientific |
3823-3512 | 12-WELL V-BOTTOM RESERVOIR | 20/pack | USAscientific |
3823-3412 | 12-WELL V-BOTTOM RESERVOIR（） | 20/pack | USAscientific |
1061-8150 | 12-CHANNEL RESERVOIR FOR AUTOMATION | 5/pack | USAscientific |
1615-5500 | SEAL-RITE® 1.5 ML MICROCENTRIFUGE TUBES | 500/bag | USAscientific |
1111-0206 | TIPONE PIPETTE TIPS IN RACKS | 10 racks of 96 (960) | USAscientific |
1111-2821 | TIPONE PIPETTE TIPS IN RACKS | 10 racks of 96 (960) | USAscientific |
1126-7810 | TIPONE FILTER TIPS | 10PACKS OF 96(960) | USAscientific |
1120-8810 | TIPONE FILTER TIPS | 10PACKS OF 96(960) | USAscientific |
1121-3810 | TIPONE FILTER TIPS | 10uL | USAscientific |
1022-0600 | 200 μL GEL-SAVER® ROUND GEL LOADING TIP | 5 racks of 204 | USAscientific |
1011-8410 | 200 μL LARGE ORIFICE TIP sterile | 10 racks of 96 (960) | USAscientific |
2512-0000 | THERMAL-LOK BLOCK FOR 15 ML TUBES | 15 ML TUBES | USAscientific |
2510-1102 | Thermal-Lok 2-Position Dry Heat Bath (Without Blocks) | ea | USAscientific |
2923-0110 | TempPlate® Sealing Foil | 50sheets/box | USAscientific |
2920-1010 | MICROPOROUS FILM | 50/box | USAscientific |
2923-5000 | NAL-96 PIERCEABLE FILM | 50 sheets/box | USAscientific |
1301-1212 | Twelve well reservoir with lid, individually wrapped, sterile, 20/pack. | 20/pack | USAscientific |
1111-1200 | TIPONE PIPETTE TIP STACKS | 960 tips | USAscientific |
9152-2200 | LABELING TAPE, 0.75IN WIDE | 2160 inch | USAscientific |
1111-2721 | TIPONE PIPETTE TIP REFILLS，1000 μL blue graduated | 10 wafers of 96 (960) | USAscientific |
1111-0706 | TIPONE PIPETTE TIP REFILLS，200 μL yellow | 10 wafers of 96 (960) | USAscientific |
1111-0506 | TIPONE PIPETTE TIP STARTER SYSTEMS | 200 uL yellow | USAscientific |
1111-2521 | TIPONE PIPETTE TIP STARTER SYSTEMS | 1000 uL blue graduated | USAscientific |
1110-3700 | TIPONE PIPETTE TIP REFILLS，10 μL XL natural graduated | 10 wafers of 96 (960) | USAscientific |
1110-3500 | TipOne Pipette Tip Starter Systems | 10 uL XL natural graduated | USAscientific |
1111-3500 | TipOne Pipette Tip Starter Systems | 10 uL natural graduated | USAscientific |
7400-2401 | Glass disruptor beads, 0.1 mm diameter, Scientific Industries SI-BG01 | each | USAscientific |
1420-9707 | SCREW CAP MICROCENTRIFUGE TUBES, SELF-STANDING, AMBER（2.0 ml棕色tube） | 500 tubes | USAscientific |
1605-0002 | Green SEAL-RITE® 0.5 ML MICROCENTRIFUGE TUBES | 1000/pack | USAscientific |
1605-0006 | Yellow SEAL-RITE® 0.5 ML MICROCENTRIFUGE TUBES | 1000/pack | USAscientific |
1605-0007 | Amber SEAL-RITE® 0.5 ML MICROCENTRIFUGE TUBES | 1000/pack | USAscientific |
1405-2600 | 0.5 ml low adhesion microcentrifuge tube, natural | 500/bag | USAscientific |
1415-2600 | 1.5 ml low adhesion microcentrifuge tube, natural 無核酸酶非粘性1.5ml試管 | bag of 500 | USAscientific |
1405-9707 | 0.5 ml self-standing screw cap tube amber | 500/pack | USAscientific |
1402-4700 | 0.2 ml PCR 8-tube FLEX-FREE strip attached clear flat caps natural 八聯(lián)排無核酸酶薄壁PCR管(0.2ml) | 盒 | USAscientific |
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